Modulation of dendritic cells by CX3CR1 and complement receptors in crescentic glomerulonephritis

Kidney dendritic cells (DCs) are central regulators in crescentic glomerulonephritis (cGN). They modulate the response of infiltrating TH cells, thereby either driving or inhibiting the progression of GN, depending on their functional state. Kidney DCs can be identified by expression of MHC II and CD11c, a component of complement receptor 4. More than 90% of renal DC also express the CD11b, a component of complement receptor 3, and the chemokine receptor CX3CR1. While the functional roles of CD11c and CD11b in the kidney are unknown, we recently showed that CX3CR1 is required for the homeostatic and inflammatory recruitment of DCs to the kidney, but not to other organs. In this proposal, we would like to clarify

1. Whether the dependence of kidney DCs on CX3CR1 depends on renal CX3CL1 expression and whether this chemokine contributes to the progression of cGN.

2. Whether pharmacological CX3CR1 blockade can attenuate cGN progression.

3. Whether the complement receptors CR3, CR4, C3aR and C5aR affect kidney DC functionality.

4. Whether these receptors affect the progression of cGN, and

5. Whether CX3CR1 and complement receptors are important for the homeostatic and inflammatory migration of DCs from the kidney to the renal LN and for induction of immunity. Our long-term aim is to explore the therapeutic potential of CX3CR1 and complement receptor inhibitors in cGN.

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